The goal of this project was to develop a laboratory protocol for the use of RAPD analysis of genetic material. We were able to obtain useful genetic markers from two of only three RAPD primers used. Monomorphic and polymorphic bands were evident in initial findings. These preliminary results suggest that RAPD analysis is an effective tool for assessing genetic variability in the eastern skunk cabbage. However, further refinements in DNA quality and the PCR conditions as well as the use of additional primers are required to accurately determine the level of genetic variation between individuals and populations.
